Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR® Phosphate Buffered Saline (PBS), High Phosphate

Supplier: VWR Chemicals

Irradiated solution, pH 7,2, for washing blots.

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VWR® Buffer Standard Solutions

Supplier: VWR Chemicals

Buffer Standard solution, Biotechnology Grade, pH value: 10,0, ±0,03, Reference temperature: 25 °C, VWR®, Bottle

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VWR®, Buffer, ammonia solution, pH 10 ready to use

Supplier: VWR Chemicals

Ideal for hardness of water determination.

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VWR®, pH electrode maintenance kit

Supplier: VWR Chemicals

The measurement of the pH value is one of the most frequently and universally made measurements in science.

What is often not fully understood is the fact that a large number of problems related to pH measurement is the
correct selection, care or maintenance of the electrodes with particular emphasis on the reference electrode.

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J.T.Baker® BAKER ANALYZED®, Buffer Solutions

Supplier: Avantor

Ready-to-use pH buffers are manufactured in large lots to stringent specifications and utilise quality control procedures to reduce lot-to-lot variability. You can eliminate the time and expense of preparation and standardisation of solutions, helping streamline high-volume analytical work in applications such as quality control.

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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

Supplier: VWR Chemicals

Sodium acetate buffer, VWR®, 3 M, pH value: 5,2

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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J.T.Baker®, Sheath fluid, 3200/3400

Supplier: Avantor

Sheath fluid, 3200/4000, J.T.Baker®

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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

Sustainable Options Available

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VWR® Buffer Solutions, Secondary Standards

Supplier: VWR Chemicals

Buffer solutions, secondary standards, pH value: 1,679, ±0,010, Reference temperature: 25 °C, VWR®, Plastic bottle

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VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.