Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™
Supplier: Honeywell Chemicals
Matrix Modifier, 2% Lithium (Li) in 1% HNO₃
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Buffer, TRIS, 1,0 M Solution, pH 9,0
Supplier: Thermo Fisher Scientific
TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: LONZA
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Enzyme storage buffer in PBS and glycerol, autoclaved
Supplier: Thermo Fisher Scientific
Enzyme Storage Buffer in PBS and Glycerol
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ADA, 0,2 M Buffer Solutions
Supplier: Thermo Fisher Scientific
N-(2-Acetimido)iminodiacetic acid monosodium salt (ADA) buffer solutions PH values 6,0 to 7,5.
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LDS-sample buffer (4X), non-reducing
Supplier: Thermo Fisher Scientific
LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.
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Phosphate-buffered saline (PBS, 10X), no potassium
Supplier: Thermo Fisher Scientific
Phosphate-buffered saline (PBS), No potassium, 10X, Liquid
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IEF Sample buffer (4X) pH 3 to 7 with lysine
Supplier: Thermo Fisher Scientific
Loading Buffer, IEF Sample Buffer (4X) with Lysine
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Laemmli SDS sample buffer, reducing (4X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Buffers, concentrated solutions, Fluka™
Supplier: Honeywell Chemicals
pH Buffer, concentrate, pH value: 5,00, ±0.02, Ampoule
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Phosphate buffer solution pH 7,00 (20 °C), Fluka™
Supplier: Honeywell Chemicals
Potassium dihydrogen phosphate / di-sodium hydrogen phosphate
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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G-Biosciences Tris-EDTA-NaCl Buffer
Supplier: G-Biosciences
Tris-EDTA-NaCl buffer solution at pH 8,0.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®
Supplier: Merck Millipore (Calbiochem)
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, triethylammonium acetate, 1 M solution, pH 7,0, Calbiochem®, Millipore®
Supplier: Merck Millipore (Calbiochem)
This buffer solution is used for the purification of chemically synthesised oligonucleotides by HPLC.
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