Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Buffers, TRIS, 0,5 M solutions

Supplier: Thermo Fisher Scientific

TRIS buffer solution, 0,5 mol/L, pH value: 8,6, Liquid

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.

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1M tris-hydrochloride buffers, Corning®

Supplier: Corning

Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.

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Phosphate, 0,2 M buffer solution, pH 7,4

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 7,4, Liquid

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Buffer, BIS-TRIS propane, 0,2 M solution, pH 6,5

Supplier: Thermo Fisher Scientific

BIS-TRIS propane buffer solution pH 6,5 (0,5 mol/l), Liquid

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M stock soln, pH 8,0

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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Phosphate, 0,5M buffer solution, pH 9,0

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH value: 9,0, Liquid

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Buffer Solutions

Supplier: Thermo Fisher Scientific

pH Buffer solution, pH value: 8,00, ±0.01, Reference temperature: 25 °C

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Acetate buffer solution, Fluka™

Supplier: Honeywell Chemicals

This pH buffer solution is a combination of sodium acetate/acetic acid in 1:1 ratio for complexometry.

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DNA gel loading buffer 6X

Supplier: LONZA

This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.

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RIPA Lysis and Extraction Buffer

Supplier: G-Biosciences

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.