Buffers

Supplier: Thermo Fisher Scientific

Glycine buffer solution, 0,2 M, pH value: 2,5, Odourless, Liquid

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Anode buffer (50X)

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

Supplier: LONZA

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 7,4

Supplier: Corning

Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer

Supplier: LONZA

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l) AccuGENE®

Supplier: Honeywell Chemicals

These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.