Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium
Supplier: Thermo Fisher Scientific
Cell culture buffer, DPBS, 1X
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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TBE running buffer (10X)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE running buffer (10X)
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Phosphate, 0,2 M buffer solution, pH 7,0
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,2 M, pH value: 7,0, Liquid
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G-Biosciences Antigen Retrieval Solution, Citrate (pH 6,0) (50X)
Supplier: G-Biosciences
Solution developed to unmask antigens in paraffin embedded tissue sections.
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HEPES-Buffered Saline, 5X Solutions
Supplier: Thermo Fisher Scientific
HEPES buffered saline, 5X, pH value: 7,0, Odourless, Liquid
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Semi Dry Blot Transfer buffer (10X)
Supplier: Thermo Fisher Scientific
Electrophoresis, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X
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Buffers, potassium phosphate, 0,5 M solutions
Supplier: Thermo Fisher Scientific
Potassium phosphate, buffer solution, 0,5 M, pH value: 7,4, Liquid
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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.
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Phosphate-buffered saline (PBS, 10X), RNase free
Supplier: Thermo Fisher Scientific
Cell culture buffer, PBS, 10 X, RNAse free
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Laemmli SDS sample buffer, reducing (6X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, reducing, 6X
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TRIS-buffered saline (TBS, 10X), with 0,5% Tween 20
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X), with 0,5% Tween 20
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TRIS-buffered saline (TBS, 10X) pH 8,0
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)



