Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Blocking buffers
Supplier: Rockland Immunochemicals
A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.
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Guanidine hydrochloride buffer solution, 6 mol/L, pH 8,7
Supplier: TCI
Guanidine Hydrochloride Buffer Solution, 6 mol/L, pH value: 8,7, TCI
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Instant Buffers (acc. to Soerensen)
Supplier: TCI
Instant Buffer S64, (acc. to Soerensen), pH value: 6.4
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Cell Lysis Buffer, Abcam
Supplier: Abcam
ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.
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Native lysis Buffer, Abcam
Supplier: Abcam
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
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Fixation buffer for microscopy and flow cytometry
Supplier: Biotium
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.
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Permeabilisation buffer
Supplier: Biotium
Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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Plasmid DNA buffers
Supplier: OMEGA BIO-TEK
Solution 1, resuspension buffer
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E.Z.N.A.® RNALock stabilizer reagent
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.
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Immunoassay Blocking (BSA Free)
Supplier: Abcam
Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
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RBC Lysis Buffer (10X)
Supplier: Tonbo Biosciences
RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.
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Electrophoresis running buffers
Supplier: G-Biosciences
There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.
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G-Biosciences Red Blood Cell Lysis Buffer
Supplier: G-Biosciences
A red blood cell lysis buffer for the isolation of white blood cells free from interference of RBCs. The optimised concentration of ammonium chloride and enhances effectively lyses red blood cells, while preserving the integrity of the leukocytes.
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Immunoassay Blocking Buffer
Supplier: Abcam
Immunoassay blocking buffer effectively preserves the conformation and activity of dried proteins in immunoassays.
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10X RIPA Buffer, Abcam
Supplier: Abcam
10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins
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