You searched for: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)
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VWR®, EDTA buffer solution pH 8,0 (0,5 mol/L) for biotechnology
Supplier: VWR Chemicals
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l) for biotechnology
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Buffer tablets according to Weise, Sigma-Aldrich®
Supplier: Merck
Ideal for preparing buffer solution according to WEISE for staining of blood smears.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: PanReac AppliChem
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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VWR®, Tris-borate-EDTA (TBE) buffer
Supplier: VWR Chemicals
TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).
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VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology
Supplier: VWR Chemicals
Tris (hydroxymethyl) aminomethane.
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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer
Supplier: VWR Chemicals
TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.
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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: VWR Chemicals
Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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VWR® UltraPure, PBS and TBS wash buffers
Supplier: VWR Chemicals
Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.
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Blocking buffers
Supplier: Rockland Immunochemicals
A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Thermo Fisher Scientific
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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Buffer, TRIS solution pH 7 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)
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VWR® Transfer Buffer Concentrate, CAPS 10X
Supplier: VWR Chemicals
For transfer of protein to membranes for sequencing or blotting.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Buffer, HEPES solution (1 mol/L)
Supplier: Cayman Chemical
HEPES buffer solution (1 mol/l)
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VWR®, Rapid Transfer buffer 10X
Supplier: VWR Chemicals
Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.
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VWR®, MOPS 10X buffer for biotechnology
Supplier: VWR Chemicals
MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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ES Buffer
Supplier: FUJIFILM WAKO CHEMICALS
The ES Buffer is an endotoxin-specific buffer that is ideal for untreated LAL.
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Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
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Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
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VWR® A.C.E.™, Sequencing buffers
Supplier: VWR Chemicals
A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Rockland Immunochemicals
EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.
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VWR®, Blocking buffer, non-fat powdered milk
Supplier: VWR Chemicals
Each pack of powdered blocking buffer makes one liter of 1X solution.
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Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)
Supplier: Rockland Immunochemicals
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
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VWR® Gentle ReView™, Stripping Buffer
Supplier: VWR Chemicals
Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.