Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Ammonia buffer solution pH10 (20 °C) for complexometry, Fluka™
Supplier: Honeywell Chemicals
Ammonia buffer
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NP-40 lysis buffer with glycerol (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific
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Imidazole-buffered saline (5X)
Supplier: Thermo Fisher Scientific
Washing buffer, Imidazole-buffered saline (5X)
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NP-40 lysis buffer
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, Thermo Fisher Scientific
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PIPES-buffered saline (5X), pH 6,5
Supplier: Thermo Fisher Scientific
PIPES-buffered saline, 5X, pH value: 6,5, Colourless, Liquid
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Buffers, MOPS, 0,5 M solutions
Supplier: Thermo Fisher Scientific
MOPS buffer solution, 0,5 mol/L, pH value: 7,5, Odourless, Liquid
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Kinase buffer I (5X)
Supplier: Thermo Fisher Scientific
Kinase buffer I (5X)
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Phosphate, 0,2 M buffer solution, pH 7,2
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,2 M, pH value: 7,2, Liquid
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Buffers, sodium phosphate, 0,2 M solutions
Supplier: Thermo Fisher Scientific
Sodium phosphate buffer solution, 0,2 mol/l, pH value: 8,5, Liquid
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Carbonate-buffered saline (5X), pH 9,5
Supplier: Thermo Fisher Scientific
Cell culture buffer, Carbonate-buffered Salin, 5X
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Phosphate, 0,5M buffer solution, pH 8,5
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,5 M, pH value: 8,5, Liquid
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Phosphate-buffered saline (PBS, 10X), with Triton® X-100
Supplier: Thermo Fisher Scientific
Washing buffer, Phosphate-buffered saline (PBS, 10X), with Triton® X-100
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Triton® X-100 lysis buffer, pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific
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IEF Cathode buffer (10X) with lysine and arginine
Supplier: Thermo Fisher Scientific
Running Buffer, IEF Cathode buffer (10X) with lysine and arginine
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Laemmli SDS sample buffer, non-reducing (4X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 4X
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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
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