Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Phosphate, 0,2 M buffer solution, pH 7,6

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 7,6, Liquid

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CHES, 0.5 M buffer solutions

Supplier: Thermo Fisher Scientific

2-(Cyclohexylamino)ethanesulfonic acid, CHES.

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TRIS-glycine native sample buffer (4X), pH 8,6

Supplier: Thermo Fisher Scientific

Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)

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Stacking buffer (4X)

Supplier: Thermo Fisher Scientific

Electrophoresis, Stacking Buffer, 4X

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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Phosphate-buffered saline (PBS, 10X), pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X)

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G-Biosciences Acetate Buffer

Supplier: G-Biosciences

Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.

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His·Bind® buffer kit, Novagen®

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

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Buffer, ACES, 0,5 M solution

Supplier: Thermo Fisher Scientific

ACES, 0,5M buffer solution, pH 7,0, Colourless, Liquid

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TE buffer, 1X, Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

Use directly to re-suspend and/or dilute purified DNA or RNA.

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TAE buffer, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.