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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

RoboSep™ Buffer 2

RoboSep™ Buffer 2

Supplier: STEMCELL Technologies

RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

Supplier: Serva

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Serva

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, sodium acetate buffer solution pH 7 (3 mol/L)

Supplier: Serva

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)

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Buffer, TRIS solution pH 8,8 (1 mol/L)

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 8,8 (1 mol/l)

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: Serva

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Molecular Biology Grade

Supplier: Serva

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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RoboSep™-C PBS/EDTA Buffer

RoboSep™-C PBS/EDTA Buffer

Supplier: STEMCELL Technologies

PBS buffer containing EDTA.

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pH Buffer

Supplier: CUSTOM MADE CHEMICALS LAB

di-Sodium hydrogen phosphate/Sodium hydroxide, 12,0 at 20 °C

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HEPES Buffer Solution (1 M)

HEPES Buffer Solution (1 M)

Supplier: STEMCELL Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum

Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum

Supplier: STEMCELL Technologies

Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.

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ALDEFLUOR™ Assay Buffer

ALDEFLUOR™ Assay Buffer

Supplier: STEMCELL Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

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Fixation Buffer

Fixation Buffer

Supplier: STEMCELL Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++

Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++

Supplier: STEMCELL Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

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EasySep™ Red Blood Cell Lysis Buffer

EasySep™ Red Blood Cell Lysis Buffer

Supplier: STEMCELL Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.

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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++

Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++

Supplier: STEMCELL Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

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Glucose hemolysing solution

Glucose hemolysing solution

Supplier: DIAGNOSTIC SYSTEMS DIASYS

For use with glucose Gluc-DH FS and glucose Gluc-DH FSC.

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Intracellular Permeabilisation Buffer (10X)

Intracellular Permeabilisation Buffer (10X)

Supplier: STEMCELL Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.

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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++

Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++

Supplier: STEMCELL Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.

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Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red

Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red

Supplier: STEMCELL Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.

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Ammonium Chloride Solution

Ammonium Chloride Solution

Supplier: STEMCELL Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.

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