You searched for: Buffers

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

Supplier: Hach

For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.

Supplier: NOVILYTIC

Set of three reagent vials for use with the Proteometer-UFT kit.

Supplier: NOVILYTIC

Fluorescent reagent portion of the Proteometer-CV starter kit. This is sold individually due to shipping and storage conditions.

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Merck

TISAB solution for fluoride determination

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

Supplier: Merck Millipore (Calbiochem‎)

Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: NOVILYTIC

Fluorescent reagent portion of the Proteometer-CV full kit. This is sold individually due to shipping and storage conditions.

Supplier: NOVILYTIC

Proteometer-CV reconstitution reagent.

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.

Supplier: G-Biosciences

Water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH.

Supplier: Rockland Immunochemicals

Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.

Supplier: Merck

TISAB III solution for fluoride determinations

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Supplier: Greisinger

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific