You searched for: Assays

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

Supplier: ENZO LIFE SCIENCES

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.

Supplier: Cayman Chemical

For measurement of LDH activity as an indicator of cell death.

Supplier: ENZO LIFE SCIENCES

This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

Supplier: Biotium

NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

Supplier: ENZO LIFE SCIENCES

The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.

Supplier: ENZO LIFE SCIENCES

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

Supplier: Abcam

Sensitive, reproducible assay for initial screening of potential glutaminase inhibitors.

Supplier: ENZO LIFE SCIENCES

This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. Activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.

Supplier: Cayman Chemical

For the measurement of creatinine levels in plasma and serum.

Supplier: Rockland Immunochemicals

These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.

Supplier: ENZO LIFE SCIENCES

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

Supplier: Cayman Chemical

Cellular assay, Cell-based assay calcein AM

Supplier: ENZO LIFE SCIENCES

The ER-ID® Green assay kit is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.

Supplier: ENZO LIFE SCIENCES

A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay

Supplier: Molecular Devices

SpectraMax® Quant™ dsDNA Assay kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations.

Supplier: Cayman Chemical

A simple and sensitive tool for assaying glycogen from tissue.

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

Supplier: ENZO LIFE SCIENCES

The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: ENZO LIFE SCIENCES

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.

Supplier: Molecular Devices

The Neurotransmitter Transporter Uptake Assay Kit provides a fast, simple and reliable fluorescence-based assay for the detection of dopamine, norepinephrine and serotonin transporter (SERT, NET and DAT, respectively) activity in expressing cells.

Supplier: Cayman Chemical

An immunometric assay for the measurement of type II collagen antibody in plasma and serum.

Supplier: ENZO LIFE SCIENCES

For the detection of Cytochrome c translocation from mitochondria into cytosol during apoptosis.

Supplier: ENZO LIFE SCIENCES

The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

Supplier: Molecular Devices

CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

Supplier: Molecular Devices

The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.