You searched for: Antibody Purification Buffers

Antibody purification buffers are necessary components in the antibody purification process, which involves selective enrichment of specific isolation of antibodies from serums, ascites fluids, or cell cultures supernatant of a hybridoma cell line. These buffers ensure high-yield, nondenaturing antibody purification, and are often provided in specialized formulations for purification of specific antibodies. Antibody purification buffers are pre-filtered and delivered ready to use, and products are available for each antibody purification method.

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Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer, antigen retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization.

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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.

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Diluent buffer, 10X concentrate, for dab membrane substrate

Supplier: Rockland Immunochemicals

Concentrate diluent buffer for DAB membrane substrate, 10X

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Antibody diluent (HAMA blocker) for ELISA

Supplier: Abcam

Antibody diluent (HAMA blocker) for ELISA is a proprietary reagent for immunoassays involving a sandwich antibody complex that are susceptible to heterophilic antibody interference.

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Binding and elution buffers for antibody purification, Pierce™

Supplier: Thermo Fisher Scientific

These buffers ensure high-yield, non denaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

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Buffer kit for antibody purification on protein A column

Supplier: Thermo Fisher Scientific

Buffer kit for antibody purification

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His·Bind® buffer kit, Novagen®

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.