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Experimentation kits, dot blot analysis

Experimentation kits, dot blot analysis

Supplier: G-Biosciences

Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

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Experimentation kits, introduction to ELISA reactions

Supplier: EDVOTEK

Students learn the basic principles of the Enzyme-linked Immunosorbent Assay (ELISA) using this precise and sensitive antibody-based detection kit.

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Experimentation kits, simulation of HIV detection by western blot (agarose-based)

Experimentation kits, simulation of HIV detection by western blot (agarose-based)

Supplier: EDVOTEK

The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.

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Experimentation kits, southern blot analysis

Experimentation kits, southern blot analysis

Supplier: EDVOTEK

This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

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Experimentation kits, DNA damage and repair

Experimentation kits, DNA damage and repair

Supplier: EDVOTEK

According to the World Health Organisation, between two and three million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

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Experimentation kits, blood-based cancer diagnostics

Experimentation kits, blood-based cancer diagnostics

Supplier: EDVOTEK

Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

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Experimentation kits, in search of the 'Kissing Disease'

Experimentation kits, in search of the 'Kissing Disease'

Supplier: EDVOTEK

Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.

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Experimentation kits, how does a doctor test for AIDS?

Experimentation kits, how does a doctor test for AIDS?

Supplier: EDVOTEK

The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.

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Experimentation kits, antigen-antibody interaction: the ouchterlony procedure

Supplier: EDVOTEK

This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

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Experimentation kits, analysis and comparision of mammalian cell types

Experimentation kits, analysis and comparision of mammalian cell types

Supplier: EDVOTEK

Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.

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Experimentation kits, morphology of cancer cells

Experimentation kits, morphology of cancer cells

Supplier: EDVOTEK

When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

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Experimentation kits, what is an epidemic and how does an infection spread?

Experimentation kits, what is an epidemic and how does an infection spread?

Supplier: EDVOTEK

Infectious agents such as bacteria and viruses can spread rapidly through a population and cause widespready disease and death. In this experiment, students use coloured solutions to simulate the spreading of a disease in the classroom.

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Experimentation kits, simulation of HIV detection by ELISA

Experimentation kits, simulation of HIV detection by ELISA

Supplier: EDVOTEK

An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

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Experimentation kits, detection of a simulated infectious agent

Experimentation kits, detection of a simulated infectious agent

Supplier: EDVOTEK

An infectious outbreak requires prompt and accurate identification of the biological agent. Often, early clinical symptoms are first identified in exposed individuals and then infectious agents are identified by lab tests. In this experiment, students transmit a simulated infectious agent (chemical dye) between classmates which is only visible under long UV light. The pattern of transmission and primary source is documented.

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Experimentation kits, antigen-antibody interactions

Experimentation kits, antigen-antibody interactions

Supplier: G-Biosciences

This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

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Poster, dependence chart

Poster, dependence chart

Supplier: 3B Scientific

Educational Materials, Physiology and Health, Application: Biology, Poster - Drugl Dependence, laminated

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Poster, alcohol dependence

Poster, alcohol dependence

Supplier: 3B Scientific

Educational Materials, Physiology and Health, Application: Biology, Poster - Alcohol Dependence, laminated

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Nicotine dependence chart

Nicotine dependence chart

Supplier: 3B Scientific

Over 30% of the world's population uses tobacco products, and 90% of that is from cigarettes. The physical effects of nicotine and the diseases it can cause, including cancer, are detailed on this anatomical poster. For such a wide spread practice education is important and this anatomy poster can help do that. This thickly laminated anatomical chart is printed on premium glossy (200 g) UV resistant paper and comes with two sided lamination (125 µm, 5,0 mil) and metal eyelets to make the chart easy to display.

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