Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

Pancreatin

Supplier: PanReac AppliChem

Pancreatin

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Recombinant collagenase H

Supplier: Apollo Scientific

From Clostridium histolyticum and produced in E. Coli and B. Megaterium. Endotoxin negative. No detectable Caseinase activity. Sterile lyophilized powder.

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Aspergillus oryzae Protease

Supplier: TCI

Aspergillus oryzae Protease

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Aspergillus niger Pectinase

Supplier: TCI

Aspergillus niger Pectinase

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Exonuclease I

Supplier: Thermo Fisher Scientific

Exonuclease I (ExoI) degrades single-stranded DNA in a 3' to 5'direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

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T4 GP32 Protein (from E.coli)

Supplier: G-Biosciences

T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.

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Soy bean beta-Amylase

Supplier: TCI

Soy bean beta-Amylase

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Recombinant uridine phosphorylase Salmonella typhimurium

Supplier: Apollo Scientific

Recombinant uridine phosphorylase Salmonella typhimurium

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Human Recombinant Palmitoyl-Protein Thioesterase 1 (from Cells)

Supplier: ProSci Inc.

Palmitoyl-protein thioesterase 1(PPT-1 for short), also known as Palmitoyl-protein hydrolase 1, belongs to the palmitoyl-protein thioesterase family.

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Proteinase K

Supplier: AGILENT

Proteinase K is a broad spectrum protease from Tritirachium album that is ideal for preparing chromosomal DNA.

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Dnase

Supplier: OMEGA BIO-TEK

For DNase digestion during RNA purification. The RNase-free DNase set provides a rapid and efficient on-column digestion of DNA during RNA purification from a wide variety of sample sources using E.Z.N.A.® RNA kits. The DNases can be efficiently removed in subsequent steps. Generally, DNase digestion is not required for RNA purified with E.Z.N.A.® RNA kits since the silica-membrance, spin-column technology efficiently removes the majority of the DNA without DNase treatment. However, complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA.

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Proteinase K solution

Supplier: PanReac AppliChem

Proteinase K is used to destruct proteins in cell lysates (tissue, cultured cells) and to liberate nucleic acids, since it very effectively digests DNases and RNases.

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Human Recombinant 15-Lipoxygenase-2 (from E. coli)

Supplier: Cayman Chemical

Two types of 15-lipoxygenase (15-LO) have been discovered and characterised, both of which metabolise arachidonic acid (AA) to produce 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). 15-LO-1 oxygenates AA at both C-15 and C-12, whereas 15-LO-2 exclusively oxygenates C-15 of AA. Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1.2,3 expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea and may play a role in the normal development of these tissues.

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Peroxidase, Horseradish, RZ3 (POD, HRP, HRPO)

Supplier: US Biological

Horseradish Peroxidase (HRP) is a hemoprotein catalysing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes.

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Bovine MSG-Trypsin™

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

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Deoxyribonuclease I

Supplier: Rockland Immunochemicals

Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.

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Human Recombinant EN-TEV Protease (from E. coli)

Supplier: Abcam

Recombinant EN-TEV Protease protein is a Tobacco etch virus Fragment protein, in the 2038 to 2279 aa range, expressed in Escherichia coli, with >=95% purity and suitable for SDS-PAGE, Size Exclusion Chromatography.

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Nuclease S1

Supplier: Rockland Immunochemicals

Nuclease S1 is an endonuclease that is active against single-stranded DNA and RNA molecules. Although its primary substrate is single-stranded, it can also introduce single-stranded breaks in double-stranded DNA-RNA or DNA-RNA hybrids. Nuclease S1 is used in the laboratory as a reagent in nuclease protection assays. It is used in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA.