You searched for: Sanger Sequencing Reagents

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT BIOQUEST

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT BIOQUEST

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: Corning

The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.

Supplier: G-Biosciences

SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.

Supplier: G-Biosciences

SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.

Supplier: G-Biosciences

SG-Glutamic-C™ is a serine endopeptidase, from S. aureus V8, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specific for the glutamyl site. Recommended buffers for fragmentation of proteins using this enzyme are 50 mM Tris-HCI, pH 8,0 or bicarbonate buffer. Highly purified preparations of SG-Glutamic-C™ are chemically modified making the enzyme both resistant to autolysis and stabilises its enzymatic activity.

Supplier: G-Biosciences

SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.

Supplier: Cytiva

AutoScreen 96 well plate consists of a 96-well filter plate containing DNA grade Sephadex™ G-50 for purification of sequencing reactions prior to analysis on MegaBACE 1000 and can be used for other size exclusion applications.