"qPCR Mastermixes"

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: Thermo Fisher Scientific

ABsolute Blue™ mastermix inkorporerer en inert blå farve med henblik på at øge kontrasten mellem reagens og plast betydeligt, hvilket gør verifikation af MasterMix-dispensering hurtig, nem og idiotsikker. Anvendelsesområder omfatter genekspression, siRNA-validering, SNP-bestemmelse og gentypebestemmelse.

Supplier: Thermo Fisher Scientific

Verso™ qRT-PCR systemet kombiner Verso™ RT enzymet med ABsolute™ Blue qPCR MasterMix for at give maksimal bekvemmelighed og alsidighed. Fås i et 1- eller 2-trins format, samt i flere pakkestørrelser, så de passer til alle applikationer og krav til flow.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: Quantabio

Dette SuperMix er en 2X koncentreret, brugsklar reaktionsblanding til realtids-qPCR, som indeholder alle komponenter, undtagen primere, prober og skabeloner. Systemet overskrider de multiplexe begrænsninger i konventionelle PCR master mixes og muliggør ikke-kompromitteret amplifikation på op til fem målsekvenser i et enkelt rør. Undertrykkelse af amplikoner med lavt antal kopier af referencemål med højt antal kopier i amplifikationen er et almindeligt problem inden for multiplexing PCR. Dette kan fordreje eller maskere den tilsyneladende repræsentation og kvantificering af målsekvenser med et lavt antal kopier. PerfeCTa® MultiPlex qPCR SuperMix leverer et dynamisk område og følsomhed til multiplex qPCR, som kan sammenlignes med det for singleplex qPCR probeassays, men uden at der er behov for begrænsende eller variable primerkoncentrationer.

Supplier: Quantabio

Syntese af cDNA og PCR-amplifikation udføres i det samme rør uden at det åbnes mellem procedurerne. Disse sæt er optimeret til at levere maksimal RT-PCR-effektivitet og -specificitet samt til muliggøre ikke-kompromitteret co-amplifikation af transkripter med lavt antal kopier ved tilstedeværelse af referencegener med et større antal kopier Som resultat heraf tilbyder qScript™ One-Step qRT-PCR-sæt forbedret følsomhed i forhold til andre kommercielt tilgængelige qPCR-reagenser. qScript™ One-Step Master Mix maksimerer både aktiviteten af revers transkriptase og PCR-enzymer, mens det samtidigt minimerer dannelse af primer-dimer. Høj specificitet sikres med AccuStart™ Taq DNA-polymerase, der er bundet af monoklonale antistoffer for overlegen hot-start. Antistofferne denaturerer irreversibelt ved opvarmning til 95 °C og frigør komplet aktivt og umodificeret Taq DNA-polymerase. qScript™ One-Step qRT-PCR-sæt fås til alle realtids-PCR-instrumentplatforme, herunder de, som kræver normalisering med ROX referencefarve.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: Stemcell Technologies

For probe-based assays and arrays.

Supplier: Quantabio

sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

Supplier: PRIMERDESIGN

[EN]OASIG LYOPHILISED 2 X QPCR MAST 150 1 * 1 Items

Supplier: PRIMERDESIGN

[EN]OASIG LYOPHIL. ONESTEP RT-QPCR MASTE 1 * 1 Each

Supplier: PRIMERDESIGN

MASTER MIX 1ML 1 * 1 Each