"derivatisation reagent"
N,O-Bis(trimethylsilyl)acetamid 25% in acetonitrile trimethylsilylating reagent, for NH₂ compounds
Supplier: TCI
N,O-Bis(trimethylsilyl)acetamid 25% in acetonitrile trimethylsilylating reagent, for NH₂ compounds
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(+)-1-(9-Fluorenyl)ethyl chloroformate opløsning ≥18 mM in acetone, LiChropur™ for chiral derivatisation, Supelco®
Supplier: Merck
(+)-1-(9-Fluorenyl)ethyl chloroformate [(+)-FLEC] is a chiral derivatizing reagent. It helps in separating enantiomers of α-amino acids, as well as helps in determining its optical purity using HPLC method.
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N,O-Bis(trimethylsilyl)trifluoracetamid med 1% trimethylchlorsilan (99:1) for GC derivatisation
Supplier: TCI
N,O-Bis(trimethylsilyl)trifluoracetamid med 1% trimethylchlorsilan (99:1)
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Carboxyl reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.
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Benzylamin ≥99.0%, LiChropur™ for GC derivatisation, Supelco®
Supplier: Merck
Benzylamin ≥99.0%, LiChropur™ for GC derivatisation, Supelco®
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TLC sprayer
Supplier: Merck
Merck TLC sprayer gør det muligt at sprøjte de fremkaldte kromatogrammer ensartet med derivatiseringsreagenser. Den er udstyret med to forskellige sprøjtedyser på 0,8 og 1,25 mm, som er optimeret til opløsninger med henholdsvis lav og høj viskositet. Den elektro-pneumatisk drevne sprayer anvender komprimeret luft, som skabes med akkumulatorkraft og induktiv opladning.
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Pentafluorobenzyl-p-toluenesulphonate ≥98.0% (by titrimetric analysis) derivatizing reagent, for GC of inorganic anions
Supplier: TCI
Pentafluorobenzyl-p-toluenesulphonate ≥98.0% (by titrimetric analysis) derivatizing reagent, for GC of inorganic anions
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N,O-Bis(diethylhydrogensilyl)trifluoroacetamide ≥85.0% (by GC) derivatizing reagent, til gas chromatografi
Supplier: TCI
Simultaneous cyclic silylene and silyl derivatizing reagent for GC
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4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride, fluorogenic, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride is a fluorescence tag for α keto acids. It is also required for the derivatisation of sialic acids in presence of dilute sulfuric acid. The method allows determination of sialic acids.
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Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™
Supplier: Thermo Fisher Scientific
Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.
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FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥90% (by HPLC), pulver suitable for protein labeling, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525 nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been proposed as a contact sensitiser.
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Iodination beads, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Iodination beads are Ø 3 mm polystyrene beads that are coated with an oxidising reagent, which provides efficient activation of 125I for protein or peptide iodination procedures.
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Desalting columns, Pierce™
Supplier: Thermo Fisher Scientific
These desalting columns are ready-to-use, disposable, gel filtration columns for separating proteins and other macromolecules from low molecular weight buffer salts and reagents.
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HPLC columns, Syncronis™ HILIC
Supplier: Thermo Fisher Scientific
The Hydrophilic Interaction Liquid Chromatography (HILIC) technique offers complementary selectivity to reversed phase. With the ability to retain highly polar and hydrophilic compounds, these columns have been developed to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns.



