buffers

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

20x PBS buffer with Tween 20, Abcam

Supplier: Abcam

20x Phosphate buffered saline (PBS) wash buffer with tween 20 (pH 6.5 - 6.9) for use during immunolabeling techniques such as immunohistochemistry (IHC). - Contains PBS, 1% sorbitan monolaurate, ethoxylated

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Extraction Enhancer Buffer 50X, Abcam

Supplier: Abcam

Abcam's Extraction Enhancer Buffer 50X is formulated for the preparation of cell or tissues extracts for use in immunoassays.

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Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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J.T.Baker® BAKER ANALYZED®, Buffer Solutions

Supplier: Avantor

Ready-to-use pH buffers are manufactured in large lots to stringent specifications and utilise quality control procedures to reduce lot-to-lot variability. You can eliminate the time and expense of preparation and standardisation of solutions, helping streamline high-volume analytical work in applications such as quality control.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

DNA wash buffer concentrate, low salt spin column was solution (makes 250 ml)

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10X Phosphate Buffered Saline, Abcam

Supplier: Abcam

10X Phosphate Buffered Saline is a buffer designed for ELISA assay development.

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VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

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Cell culture salts, salt solutions and buffers

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Cell Lysis Buffer, Abcam

Supplier: Abcam

ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

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G-Biosciences Red Blood Cell Lysis Buffer

Supplier: G-Biosciences

A red blood cell lysis buffer for the isolation of white blood cells free from interference of RBCs. The optimised concentration of ammonium chloride and enhances effectively lyses red blood cells, while preserving the integrity of the leukocytes.