buffers

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

Supplier: NOVILYTIC

The Proteometer-CV is designed for the scientific development of mAbs, bi-specifics, or fusion proteins with a human Fc region, regardless of their stage in the development continuum. This kit offers an alternative to traditional charge variant methods that require Protein A.

Supplier: Thermo Fisher Scientific

1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-CV kit.

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

Supplier: NOVILYTIC

The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

Supplier: Merck Millipore (Calbiochem‎)

Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.

Supplier: Honeywell Chemicals

TISAB according to DEV D4.

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

Supplier: G-Biosciences

Solution developed to unmask antigens in paraffin embedded tissue sections. 

Supplier: Merck Millipore (Novagen)

BugBuster® proteinekstraktionsreagens er formuleret til nænsom ødelæggelse af cellevæggen i E. coli, hvilket frigører opløseligt protein. Et simpelt, hurtigt og økonomisk alternativ til mekaniske metoder som French Press eller ultralydslysering. Til frigivelse af udtrykt målprotein i præparation til oprensning eller andre opgaver. Formuleringen benytter sig af en detergentblanding som er i stand til at perforere cellevæggen uden af denaturere det opløselige protein.