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buffers

Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

 
 
Sodium acetate, Corning® 3M™

Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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pH buffers, ready to use

pH buffers, ready to use

Supplier: WTW

Every bottle has its own lot reference with the expiration date.

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Proteometer-CV starter kit part 2/2 reagent (60 assays)

Proteometer-CV starter kit part 2/2 reagent (60 assays)

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-CV Starter Kit.

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Ab buffer kit

Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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Nuclear and cytoplasmic extraction reagents, NE-PER

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

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Sodium bicarbonate, Corning®

Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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Protexidase™, peroxidase protection buffer, Immuno™, MP Biomedicals

Supplier: MP Biomedicals

A peroxidase protective buffer which stabilises HRP conjugates. It has 500 ml of PBS, pH 7,2, containing 0,5% BSA, antimicrobial preservatives and proprietary stabilising compounds.

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Proteometer-CV mobile phase A salts

Proteometer-CV mobile phase A salts

Supplier: NOVILYTIC

5 sealed salt packets specifically formulated for use with Proteometer-CV kit.

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Proteometer-L consumables kit

Proteometer-L consumables kit

Supplier: NOVILYTIC

The Proteometer-L Kit transforms any LC into an analytical system capable of identifying and quantifying titer and relative aggregate content in crude culture filtrate in <10 minutes, without sample preparation.

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Proteometer-L starter kit part 2/2 reagent (200 assays)

Proteometer-L starter kit part 2/2 reagent (200 assays)

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-L Starter Kit.

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

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Blocking reagents, Blotto

Blocking reagents, Blotto

Supplier: Rockland Immunochemicals

Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.

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Proteometer-L fluorescent reagent

Proteometer-L fluorescent reagent

Supplier: NOVILYTIC

7 vials of the Proteometer-L fluorescent reagent specifically formulated for use with Proteometer-L kit.

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Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™

Supplier: Honeywell Chemicals

TraceSELECT™, for flame atomic absorption spectrometry.

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