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buffers

Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

 
 
20X SSPE, liquid, Corning®

20X SSPE, liquid, Corning®

Supplier: Corning

20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.

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Buffer, potassium acetate, 1 M aq, soln, pH 7,5, RNAse free

Supplier: Thermo Fisher Scientific

Cloning Buffer, Potassium acetate, 1M aq, solution, pH 7,5, RNAse free

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His buffer kit

His buffer kit

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

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Sodium Cacodylate, 0,1 M Buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Liquid.

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His·Bind® buffer kit, Novagen®

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

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IPG buffers

IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

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TISAB solution for Fluoride Determination, Supelco®

TISAB solution for Fluoride Determination, Supelco®

Supplier: Merck

TISAB solution for fluoride determination

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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ISA (ionic strength adjustment solution: 1 M KCl), Supelco®

Supplier: Merck

Solution used to establish a constant ionic strength in a sample solution containing e.g. calcium for det. with ISE.

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Buffer Capsules

Supplier: Greisinger

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.

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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

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HyClone™ Earle's 1X Balanced Salt Solution (EBSS): Solution

HyClone™ Earle's 1X Balanced Salt Solution (EBSS): Solution

Supplier: HyClone products (Cytiva)

Balanced salt solution for cell culture applications.

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TISAB I Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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Protein blocking solution, BLOK™ BLOTTO

Protein blocking solution, BLOK™ BLOTTO

Supplier: G-Biosciences

BLOK™ BLOTTO is a 5% novel modified milk protein solution used for Westerns, ELISA and dot blot blocking.

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Phosphate buffer solution pH 7,00 (20 °C), Fluka™

Supplier: Honeywell Chemicals

Potassium dihydrogen phosphate / di-sodium hydrogen phosphate

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TISAB II Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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