buffers

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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HEPES protein extraction buffer (5X)

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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G-Biosciences Phosphate Buffer (200 mM Each)

Supplier: G-Biosciences

Water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH.

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Hybridisation buffers, formamide-free, HYB-LINK™

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

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Buffers, PIPES, 1,0 M solutions

Supplier: Thermo Fisher Scientific

Liquid

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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GST Buffer Kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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Buffer, triethylammonium acetate, 1 M solution, pH 7,0, Calbiochem®, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

This buffer solution is used for the purification of chemically synthesised oligonucleotides by HPLC.

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Phosphate-buffered saline (PBS, 10X), pH 7,6

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X

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Buffer, potassium acetate, 1 M aq, soln, pH 7,5, RNAse free

Supplier: Thermo Fisher Scientific

Cloning Buffer, Potassium acetate, 1M aq, solution, pH 7,5, RNAse free

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M solution, pH 7,4, RNase free

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.

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Buffer, HEPES solution (1 mol/L), Corning®

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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His buffer kit

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

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TRIS-buffered saline (TBS, 10X, high salt) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X, high salt)

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DIGE buffer kit

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

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NucleoSpin® Kits for Genomic DNA Extraction

Supplier: MACHEREY-NAGEL

Combining selective purification technologies and chemistries, these are a comprehensive range of specialised DNA isolation kits for a broad range of diversified starting materials. The resulting high quality DNA serves as excellent basis for following demanding downstream applications.