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buffers

Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

 
 
Protein extraction kit, Insect PE LB™

Protein extraction kit, Insect PE LB™

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

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Triethylammonium acetate buffer, BioUltra, volatile buffer, Fluka®

Supplier: Honeywell Chemicals

Triethylammonium acetate buffer, 1,0 M in H₂O, pH-værdi: 7,0

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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Non-animal sera protein blocking Agent, FISH-Blocker™

Non-animal sera protein blocking Agent, FISH-Blocker™

Supplier: G-Biosciences

FISH-Blocker™ uses a fish protein as the primary blocking agent to eliminate cross reactivity. Using a non mammalian protein eliminates or minimises the interaction of antibodies raised in mammals. It is offered either in TBS (Tris-buffered saline) or in PBS (phosphate-buffered saline) at pH 7,5.

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Hybridisation buffer, SSPE 20X, AccuGENE™

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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Phosphate-buffered saline (PBS, 10X), RNase free

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X, RNAse free

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Buffers, potassium phosphate, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Potassium phosphate, buffer solution, 0,5 M, pH-værdi: 7,0, Liquid

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TE buffer, 1X, Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

Use directly to re-suspend and/or dilute purified DNA or RNA.

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TAE buffer, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.

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Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium

Supplier: Thermo Fisher Scientific

Cell culture buffer, DPBS, 1X

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Buffer, HEPES solution pH 7,3 (1 mol/L), Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

HEPES buffer solution pH 7.3 (1 mol/l)

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M stock soln, pH 8,0

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)

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Gel loading solution, 10X

Supplier: EDVOTEK

1 ml concentrate for 200 samples.

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Buffers, BIS-TRIS propane, 0,2 M solutions

Supplier: Thermo Fisher Scientific

2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxy methyl)propane-1,3-diol]

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M solution, pH 7,4, RNase free

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)

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Blocking solution, BLOK™ Casein

Blocking solution, BLOK™ Casein

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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