buffers

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

Protein extraction kit, mammalian cell PE LB™

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, HEPES solution (1 mol/L), ULTROL Grade, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

HEPES buffer solution (1 mol/l)

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GTE buffer

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Citrat, pH-værdi: 2,00, ±0.02, Referencetemperatur: 20 °C, Fluka™, 6×1 L

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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TRIS-glycine-SDS running buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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Buffer solution for chlorine determination

Supplier: Hach

For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.

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TRIS-glycine native sample buffer (4X), pH 8,6

Supplier: Thermo Fisher Scientific

Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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CHES, 0.5 M buffer solutions

Supplier: Thermo Fisher Scientific

2-(Cyclohexylamino)ethanesulfonic acid, CHES.

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Buffers, PIPES, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Liquid

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AccuGENE® PBS Buffers

Supplier: LONZA

AccuGENE™ Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M stock soln, pH 8,0

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)

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Protein extraction kit, Insect PE LB™

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.