buffers

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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BIS-TRIS, 0,2 M Buffer Solutions

Supplier: Thermo Fisher Scientific

BIS-TRIS buffer solution, 0,2 M, pH-værdi: 6,5, Colourless, Odourless, Liquid

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TRIS-glycine-native running buffer (10x), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-native running buffer (10x)

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G-Biosciences Acetate Buffer

Supplier: G-Biosciences

Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M solution, pH 8,0, RNase-free

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)

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Buffers, glycylglycine, 0,2 M solutions

Supplier: Thermo Fisher Scientific

Glycylglycine, buffer solution, 0,2 M, pH-værdi: 8,0, Colourless, Odourless, Liquid

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TE buffer, 1X, ready to use, pH 8,0, low EDTA, Molecular Biology Grade, Affynmetrix/USB

Supplier: Thermo Fisher Scientific

TE buffer with low EDTA. Use directly to re-suspend and/or dilute purified DNA or RNA.

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TE buffer, pH 7,4, RNAse free

Supplier: Thermo Fisher Scientific

TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.

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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Citrat, pH-værdi: 2,00, ±0.02, Referencetemperatur: 20 °C, Fluka™, 6×1 L

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Phosphate, 0,5M buffer solution, pH 7,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH-værdi: 7,5, Liquid

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TAPS, 0,2 M buffer soln., pH 8,5

Supplier: Thermo Fisher Scientific

TAPS buffer soln, 0,2 M, pH-værdi: 8,5, Liquid

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POPSO, 0.2 M buffer solution, pH 8.0

Supplier: Thermo Fisher Scientific

POPSO buffer solution, 0,2 M, pH-værdi: 8,0, Liquid

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1M tris-hydrochloride buffers, Corning®

Supplier: Corning

Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.

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Protein extraction kit, mammalian cell PE LB™

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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TE buffer, (20X), pH 8,0, autoclaved

Supplier: Thermo Fisher Scientific

TE buffer concentrate, autoclaved, 20X, pH-værdi: 8,0, Liquid

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Solubilisation buffer, 2D-Xtract™

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.