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buffers

Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

 
 
VWR®, Phosphate Buffered Saline (PBS), pH 7,4

VWR®, Phosphate Buffered Saline (PBS), pH 7,4

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.

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G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)

G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)

Supplier: G-Biosciences

The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

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pH buffers, colour coded, reference standards, Supelco®

pH buffers, colour coded, reference standards, Supelco®

Supplier: Merck

pH Buffer solution, colour coded, pH-værdi: 4,00, ±0,01, Referencetemperatur: 25 °C, rød, Flaske, 6×500 ml

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Optimizer Buffer™ for optimal cross-linking and modification

Optimizer Buffer™ for optimal cross-linking and modification

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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Tris-EDTA (TE) buffer solution for molecular biology, pH 7,4, BioUltra, Supelco®

Supplier: Merck

Tris-EDTA buffer solution has been used for the dilution of DNA samples.[1] It has been used for the elution of purified vector.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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Kinase Assay Buffer I, Abcam

Supplier: Abcam

Stock solution of buffer used for assaying kinase protein.

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Blocking Buffer 10X

Blocking Buffer 10X

Supplier: Abcam

Blocking Buffer 10X is part of the reagents, controls and accessories range.

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Buffer solutions, according to EP and USP, Certipur®

Supplier: Merck

Buffer solutions intended for extremely precise calibration, qualification and monitoring of pH instruments, and for testing other buffer solutions.

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Buffer solutions, ready to use

Supplier: Thermo Fisher Scientific

pH Buffer solution, Fosfat, pH-værdi: 7,00, ±0,02, ufarvet

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Kinase Dilution Buffer III, Abcam

Supplier: Abcam

Solution of buffer used for diluting kinase protein.

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RIPA Buffer, Abcam

Supplier: Abcam

A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay.

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Gram Negative lysis buffer

Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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ChIP Buffer, Abcam

Supplier: Abcam

ChIP Buffer for ab185913

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SDS-PAGE Sample Buffer, 2X, (2-Mercaptoethanol-free) for Electrophoresis

Supplier: TCI

SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

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