buffers
Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.
VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade
Supplier: VWR Chemicals
Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)
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Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
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Phosphate buffer solution, BioUltra, Supelco®
Supplier: Merck
Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH-værdi: 7,2
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Phosphate Buffer Solution
Supplier: SIGMA ALDRICH MICROSCOPY
Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.
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Trizmal™ Buffer, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.
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10X Tris-EDTA buffer pH 9,0
Supplier: Novus Biologicals
10X Tris-EDTA buffer pH 9.0.
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WB blocking buffer kit, TrueBlack®
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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VWR®, Tris buffer solution, pH 9 (1 mol/L), ultrapure
Supplier: VWR Chemicals
Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)
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Permeabilisation buffer
Supplier: Biotium
Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0
Supplier: Rockland Immunochemicals
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0
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EDTA Buffer, pH 8,5, 10X, Antigen Retriever
Supplier: SIGMA ALDRICH MICROSCOPY
Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer
Supplier: VWR Chemicals
TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.
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VWR®, SSC buffer, 20X, ultra-pure grade
Supplier: VWR Chemicals
SSC buffer is a hybridisation buffer for Northern and Southern blots.
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Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
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