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buffers

Buffere

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

 
 

SSC buffer, 20X concentrate, pH 7,0

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Rockland Immunochemicals

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.

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Opti-Klear™ Live Cell Imaging Buffer 5X, Abcam

Supplier: Abcam

Hepes based imaging buffer formulated to lower background fluorescence and maintain proper pH and osmolarity for hours at 37°C. ab275939 provides an energy source and preserves fluorescent signals.

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Opti-Klear™ Live Cell Imaging Buffer 1X, Abcam

Supplier: Abcam

Hepes based imaging buffer formulated to lower background fluorescence and maintain proper pH and osmolarity for hours at 37°C. ab275938 provides an energy source and preserves fluorescent signals.

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Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

Supplier: Merck

Materialer til præcis kalibrering og kontrol af pH-instrumenter.

    
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: PanReac AppliChem

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)

Supplier: G-Biosciences

Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Rockland Immunochemicals

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

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DNA/RNA clean-up buffers

Supplier: OMEGA BIO-TEK

CP buffer, Omega Bio-Tek

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EDTA, 0.5 M solution, pH 8.0, for biotechnology

EDTA, 0.5 M solution, pH 8.0, for biotechnology

Supplier: VWR Chemicals

Cloning Buffer, EDTA, 0,5 M solution

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SDS-Page running gel buffers, 10X concentrate

SDS-Page running gel buffers, 10X concentrate

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.

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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer) pH 8,3

Supplier: PanReac AppliChem

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer) pH 8,3

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VHB DNA Washing Buffer

Supplier: OMEGA BIO-TEK

VHB buffer must be diluted with ethanol before use.

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Buffer solutions, Certipur®, colour coded, Supelco®

Buffer solutions, Certipur®, colour coded, Supelco®

Supplier: Merck

Materialer til præcis kalibrering og kontrol af pH-instrumenter.

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G-Biosciences Tris Buffered Saline, 10X

Supplier: G-Biosciences

TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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Wash buffer, Pierce™

Wash buffer, Pierce™

Supplier: Thermo Fisher Scientific

These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.

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