buffers

Buffere modstår pH-ændringer og beskytter prøver, når der tilsættes syrer eller baser. Pulver- og flydende former sporer effektivt proteiner under elektroforeseisolering og -separation. Saltbufferne kan også bruges til at kalibrere og standardisere pH-metre for nøjagtige aflæsninger. Blokerende stoffer forhindrer antistofbinding til membranfri steder, der ville producere en interfererende baggrund. Buffere kan fås i flydende eller dehydrerede formater i forskellige koncentrationer, der passer til ethvert behov.

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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Buffer HF-Improved

Supplier: Thermo Fisher Scientific

Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Buffer solution pH 5, Acetate Buffer

Supplier: Thermo Fisher Scientific

Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., pH-værdi: 4,98 - 5,02 (20 °C)

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VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

RNA Wash Buffer I

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TE Buffer (1X) pH 8,0 for Molecular Biology

Supplier: Apollo Scientific

Cloning Buffer, TE buffer (1X)

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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®

Supplier: Merck

1M Buffer, also for use in ion-exchange chromatography and electrophoresis.

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VWR®, pH buffer capsules

Supplier: VWR Chemicals

pH buffer capsules, Capsules, pH-værdi: 7,00, ±0,02, VWR®, grøn, Plastflaske, 5 plastic tubes, 10 capsules/tube

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology

Supplier: VWR Chemicals

137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer

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Instant Buffers (acc. to Soerensen)

Supplier: TCI

Instant Buffer S70, (acc. to Soerensen), pH-værdi: 7.0

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Electrophoresis running buffers

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.