378 Resultater for: "VARLP080520VARI00001"

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Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B. Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

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Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa's staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.

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Bromothymol Blue sodium salt has been used as a pH indicator in the yeast growth media (YPD, yeast extract peptone dextrose).

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Eosin Y, also known as Eosin yellowish is a widely used xanthene dye. It is a counterstain that is used to distinguish between various connective tissue fibers and matrices, and the cytoplasm of different types of cells. Eosin in combination with hematoxylin is useful to demonstrate the general histological architecture of a tissue.

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Bromothymol blue has been used for freshwater pH measurements over a broad range of buffer intensities.

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Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5 kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

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Patent Blue VF has been used in combination with 99mTc phytate to detect sentinel lymph node (SLN) in early stage cervical cancer patients. To detect heat shock protein (HSP) gp96 induced draining lymph nodes (LNs) in mice and to detect the effect of Asn-Gly-Arg (NGR)-tumor necrosis factor (TNF) on tumor perfusion in murine model. It is also used a diagnostic assay manufacturing, hematology, histology.

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Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall. Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

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Sunset yellow FCF is a food dye, which is is substantially used in various food preparations. It is a orange-red coloured crystal, that is soluble in water and ethanol. It is used in treating bone metabolic diseases, dermatological disorders, respiratory illness etc. It can alter the functional responses of splenocytes at non-cytotoxic dose. It promotes immune suppression. Relative expression of surface receptors in T and B cells can be altered by sunset yellow FCF.

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Basic fuchsin is a triaminotriphenylmethane dye that is widely used to stain muscle, mitochondria, and collagen. It is generally a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520).

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ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

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Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

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Readily polymerizes in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells, ErbB4 in neuroblastoma cells, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase Cγ in PC12 cells. Inhibits apoptosis. It prevents down-regulation of Ca²⁺ -impermeable GluR2 receptors and inhibits calpain, a Ca²⁺ -activated protease that is activated during apoptosis.

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Wright stain, a modified romanowsky stain, is intended for differential staining of the cellular elements of blood. It comprises polychrome methylene blue and eosin B. Upon staining blood films with wright stain, the white blood cell nucleus and cytoplasm display characteristic blue or pink hues.

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Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.

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Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.

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Phenol Red Sodium Salt (PRSS) is a water-soluble anionic fluorescent dye, that possesses an extra Na ion attached with the SO3 group compared to phenol red. It is intended for use in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6,4 or below and a red color at a pH of 8,2 and above.

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Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

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Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial's reagent for the determination of RNA, DNA, and hexoses.

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ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

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Phloroglucinol (phlo) is a phenol derivative that shows a cytoprotective effect from oxidative damage by enhancing the activity of cellular catalase. It can also be used to prepare synthetic analogs of A-type proanthocyanidins (PACs) such as 2,8-dioxabicyclo nonane derivatives by reacting with the corresponding flavylium salts. Phlo can react with benzaldehyde derivatives to form phloroglucinol-based microporous polymeric organic frameworks (phlo-POF) with potential applications in ion exchange and gas adsorption. Phloroglucinol is an antispasmodic and a derivative of phenol.

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Bromophenol blue has been used in the sample buffer prepared for western blotting. A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

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Trypan blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

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Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

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Brilliant Blue R has been used in staining of gels after electrophoresis. It has also been used to determine protein in the protein bodies. For detection of protein bands following electrophoresis.

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Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

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Brilliant Blue G has been used for determining the urine protein concentration. It has been used for the protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used as a P2X7R (purinergic receptor) antagonist.

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Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.

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Chrysin has been used to study the effect of bioflavonoids on the inhibition of ATP synthase in Escherichia coli. It has been used to study the effect of Chrysin on eIF4E (eukaryotic translation initiation factor 4E) expression in AGS cell (human gastric carcinoma cell) line. It has been used to study the effect of chrysin on human ovarian cancer cells.

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Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.