"USLP070318BIOP00035"
dNTP set, MP Biomedicals
Supplier: MP Biomedicals
Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial.
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dNTP sets (dA, dC, dG, dT) 100 mM solutions
Supplier: G-Biosciences
Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. Also available separately.
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High Purity rNTPs, Cytiva
Supplier: Cytiva
Easy to use, time saving solutions for in vitro transcription or for reactions requiring rNTPs of high purity.
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E.Z.N.A.® RNALock stabilizer reagent
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.
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PerfeCTa® MultiPlex qPCR SuperMix, low ROX
Supplier: Quantabio
PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.
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dNTP mix
Supplier: Quantabio
The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.
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qPCR SuperMixes, PerfeCTa®
Supplier: Quantabio
PerfeCTa® qPCR SuperMixes er 2X koncentrerede, brugsklare reaktionsblandinger, som indeholder alle komponenter undtagen primere, probe og qPCR-skabelon.
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AccuStart™ II PCR SuperMix
Supplier: Quantabio
AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
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Taq DNA polymerase
Supplier: G-Biosciences
Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product
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PerfeCTa® qPCR ToughMix®
Supplier: Quantabio
PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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qPCR SuperMix, PerfeCTa® MultiPlex
Supplier: Quantabio
Dette SuperMix er en 2X koncentreret, brugsklar reaktionsblanding til realtids-qPCR, som indeholder alle komponenter, undtagen primere, prober og skabeloner. Systemet overskrider de multiplexe begrænsninger i konventionelle PCR master mixes og muliggør ikke-kompromitteret amplifikation på op til fem målsekvenser i et enkelt rør. Undertrykkelse af amplikoner med lavt antal kopier af referencemål med højt antal kopier i amplifikationen er et almindeligt problem inden for multiplexing PCR. Dette kan fordreje eller maskere den tilsyneladende repræsentation og kvantificering af målsekvenser med et lavt antal kopier. PerfeCTa® MultiPlex qPCR SuperMix leverer et dynamisk område og følsomhed til multiplex qPCR, som kan sammenlignes med det for singleplex qPCR probeassays, men uden at der er behov for begrænsende eller variable primerkoncentrationer.



