"Spectrophotometer"

Supplier: G-Biosciences

This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: VWR Collection

[EN]FLOURESCENCE FREE CELL 1 * 1 Items

Supplier: EDVOTEK

When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

Supplier: Hach

[EN]DUST COVER FOR DR3900 RFID SPECTROPH 1 * 1 Items

Supplier: BIOCHROM

[EN]SPECTROPHOTOMETER BIOWAVE 3+ 1 * 1 Items

Supplier: Hach

[EN]SLIDING LID ANTIDAZZLE SHIELD 1 * 1 Items

Supplier: Lovibond Tintometer

SPECTROPHOTOMETER RT 500 1 * 1 Items

Supplier: DIDALAB

[EN]SPECTROPHOTOMETER BASIC 1 * 1 Items

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Supplier: BIOCHROM

[EN]SPECTROPHOTOMETER BIOWAVE 3+W/BLUETO 1 * 1 Items

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: BIOCHROM

[EN]SPECTROPHOTOMETER BIOWAVE 3+W/PRINTE 1 * 1 Items

Supplier: Lovibond Tintometer

[EN]SPECTROPHOTOMETER RT 520 1 * 1 Items

Supplier: Cole-Parmer

[EN]SPECTROPHOTOMETER GENOVA BIO 1 * 1 Items

Supplier: Cole-Parmer

[EN]SPECTROPHOTOMETER MODEL 6300 1 * 1 Items