"SIGMA ALDRICH MICROSCOPY"

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Cibacron Brilliant Yellow 3G-P, (Reactive yellow 2)

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Thionin(acetate), Sigma-Aldrich®

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Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. Fluorescent wavelengths λex 492 nm; λemm 517 nm in 0.1 M Tris, pH 8.0.

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Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

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Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

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Suitable for use as a protease substrate.

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Used in procedure HT100 for the toning of sections stained with the silver methanamine procedure.

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Nigrosin, alcohol soluble. Nigrosin is a black colored powder. It is widely used to manufacture ink and shoe polish. It is also employed for dyeing leather, wood and textiles. In addition, alcohol soluble nigrosin dye is used in carbon papers, typewriter and ribbons. It is used as an ingredient in rubber repairing lacquer.

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4,4′-Methylenebis(N,N-dimethylaniline) is an intermediate in dye manufacture and works as a reagent for the determination of lead. It is suitable for the qualitative assay of cyanogens.

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3-Methoxydiphenylamine can be used in the preparation of 3- methoxydiphenylamine-4-diazonium salts (MDP-4-DS) and the corresponding diazoresins.[1] Diazonium salts and diazoresins find applications in dyes, stains, indicators, general laboratory use, and industrial applications.

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3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4"-disulfonic acid sodium salt is also known as ferrozine.

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Alcianblue 8GX solution has been used as a staining dye for chondrocytic, osteoblastic and adipocytic differentiation. It has also been used for the staining of sections in immunohistochemistry.

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Reichstein's substance S ≥98%

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Alcian Blue, pyridine variant, Dye content ≥85 % (Alcian Blue-tetrakis(methylpyridinium) chloride)

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Leukocyte Alkaline Phosphatase (LAP) kits are intended for the semi-quantitative demonstration of alkaline phosphatase activity in white blood cells.

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Fontana-Masson staining is used for the demonstration of argentaffin substances such as melanin, argentaffin granules and some neurosecretory granules. Staining patterns may be useful in cases of carcinoid tumors and melanoma.