EDTA buffer, pH 8.5, 10×, antigen retriever, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.
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Acid Phosphatase, Leukocyte (TRAP) Kit Kit formulated with all liquid reagents, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Kit is used to demonstrate acid phosphatase and tartrate resistant acid phosphatase (TRAP) in blood, bone marrow and tissue touch preparations.
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Carmine, pulver, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Carmine is a red compound and is generally used with aluminum, iron and other metal salts to enhance its activity. During smear preparation, it is required for chromatin staining in fresh material. In presence of aluminum chloride, it is used for the staining of glycogen.
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Hæmatoxylin modified solution (Harris), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.
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Hæmatoxylin opløsning Gill III, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Gill formulation No. 3 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time. Used with hematoxylin and eosin staining.
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Giemsa's farve modified, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
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6-Aminofluorescein (isomer II), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed 'buckysomes'. 6-aminofluorescein dissolved in 0,1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110 °C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein. 6-Aminofluorescein has been used to determine its ability to potentiate the antiviral activity of poly r (A-U).
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Reticulocyte stain, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Utilizes New Methylene Blue for the identification of reticulocytes in blood films.
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Nile blue A, certified by the Biological Stain Commission, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Nile Blue A has been used for the staining of eggs before transferring them to the hosts. It has been used for the staining of cells.
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Fast Red Violet LB Salt ≥90% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fast Red Violet LB Salt has been used for the detection of tartrate-resistant acid phosphatase (TRAP) in tissue sections.
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Fluorescent brightener 28, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps.
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Hæmatoxylin opløsning (Mayer), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
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Sudan II 90% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Sudan II has been used to determine its presence in commercial spices such as hot chilli food samples (LC–ESI–MS/MS).
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Silver Protein
Supplier: SIGMA ALDRICH MICROSCOPY
Protargol silver impregnation and neuronal selectivity for light and electron microscopic observations.
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Reticulum Stain Kit acc. to Gordon & Sweets, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
The kit is based upon the method of Gordon and Sweets.
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Amyloid Stain, Congo Red Kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Amyloid protein is detected in tissue with Congo red, a metachromatic stain. Staining is intensified by pretreatment of tissue with alkaline sodium chloride.
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Tartrazine, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Yellow counterstain suitable for a wide range of histological staining procedures.
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Papanicolaou's solution EA 65, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.
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Silver methenamine
Supplier: SIGMA ALDRICH MICROSCOPY
Intended for use in the silver stain (modified GMS) procedure.
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Formalin 10% Histologisk fikseringsvæske, neutral buffered, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Formalin solution (10%, neutral buffered) is a widely used fixative for samples processed for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
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Olie rød O 0,5% in isopropanol (IPA), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.
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5-CFDA (5-Carboxyfluorescein diacetate), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.
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Sudan Orange G 85% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Sudan orange G belongs to the group of azo dyes, which are synthetic organic colorants. Members of azo dyes contain chromophoric azo groups (-N-N-).
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2,3,5-Triphenyltetrazoliumchlorid ≥98% (by HPLC), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
2,3,5-Triphenyltetrazoliumchlorid ≥98% (by HPLC), Sigma-Aldrich®
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Dihydrofluorescein diacetate ≥97%, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Dihydrofluorescein diacetate,97% (Diacetyldihydrofluorescein)
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Thymol blå natriumsalt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Thymol blå natriumsalt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®
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Azure II, pulver, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Azure II has been used in the Richardson′s stock solution for the staining of lysosomal glycogen. It has also been used in the periodic acid schiff staining procedure for the staining of glycogen, nuclei and cytoplasm.
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Alizarin red S (sodium salt), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Alizarin red S (sodium salt), Sigma-Aldrich®
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Aurintricarboxylsyre ≥85%, pulver, Practical grade for titration, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Readily polymerizes in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells, ErbB4 in neuroblastoma cells, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase Cγ in PC12 cells. Inhibits apoptosis. It prevents down-regulation of Ca²⁺ -impermeable GluR2 receptors and inhibits calpain, a Ca²⁺ -activated protease that is activated during apoptosis.
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Bromthymol blå natriumsalt, pulver, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Bromothymol Blue sodium salt has been used as a pH indicator in the yeast growth media (YPD, yeast extract peptone dextrose).