1073 Resultater for: "SIGMA ALDRICH MICROSCOPY"

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Formalin solution, neutral buffered, 10% is the most commonly used fixative for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

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Intended for use in the Gram Stain Procedure No. HT90.

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Lissamine Green B has been used in blood-brain barrier (BBB) studies and is used to study the its effect on herpes simplex virus 1 (HSV-1) corneal infection in animals.

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Fluorescent probe for hydrophobic (non-polar) regions in proteins and nucleoproteins.

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Dye content - ≥85%.

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4,4′-Methylenebis(N,N-dimethylaniline) is an intermediate in dye manufacture and works as a reagent for the determination of lead. It is suitable for the qualitative assay of cyanogens.

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Hematein, for microscopy (Hist.).

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Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.

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Basacryl Red GL, Dye content 19 % (Basic Red 29)

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When immobilised using an insoluble porous support matrix such as agarose, Cibacron Blue 3GA is used for affinity chromatography purification of enzymes. This affinity has been attributed to a structural similarity between the dye and the natural ligands for proteins with cofactor binding domains.

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Fontana-Masson staining is used for the demonstration of argentaffin substances such as melanin, argentaffin granules and some neurosecretory granules. Staining patterns may be useful in cases of carcinoid tumors and melanoma.

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Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Aniline blue is used to stain collagen fibers in tissue sections using the Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. It has also been used to stain and visualize glycosylated proteins.

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(Ammoniated ruthenium oxychloride)

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Histology slides with paraffin embedded section of human intestine containing argentaffin granules. Suitable for use with Fontana-Masson stain kit (Procedure No. HT200).

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Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

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Wright Stain solution, for microscopy (Eosin Methylene blue solution according to Wright; Wright's eosin methylene blue)

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Thymol iodide, (4,4'-Bis(iodooxy)-2,2'-dimethyl-5,5'-bis(1-methylethyl)-1,1'-biphenyl)

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Spin trap used in conjunction with Fe²⁺ to detect NO in brain, kidney, liver, and other tissues. Simultaneous direct measurement of NO* and pO₂ has been reported. A copper chelator, it has been used to quantitate copper in biological materials by ESR and is an inhibitor of superoxide dismutase, ascorbate oxidase, and other enzymes. Also used for colorimetric determination of small quantities of arsenic. Silver diethyldithiocarbamate has been used in cell culture. Also used for colorimetric determination of small quantities of arsenic. Spin trap used in conjunction with Fe²⁺ to detect NO in brain, kidney, liver, and other tissues. Simultaneous direct measurement of NO* and pO₂ has been reported. A copper chelator, it has been used to quantitate copper in biological materials by ESR and is an inhibitor of superoxide dismutase, ascorbate oxidase, and other enzymes.

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bisBenzimide H 33258 has been used for nuclear staining in cells. bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.

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Mordant Blue 9, Dye content 50 % (6-(5-Chloro-2-hydroxy-3-sulfophenylazo)-5-hydroxy-1-naphthalenesulfonic acid disodium salt; 6-(5-Chloro-2-hydroxy-4-sulfonphenylazo)-5-hydroxy-1-naphthalene-sulfonic acid sodium salt)

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Orange II sodium salt,(Certified by the Biological Stain Commission), Dye content ≥85 % (4-(2-Hydroxy-1-naphthylazo)benzenesulfonic acid sodium salt; Acid Orange 7; Acid Orange A; Orange II; Tropaeolin 000 No. 2).

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o-Cresolphthalein, Sigma-Aldrich®

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A cell-permeable generator of singlet oxygen.

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N,N-Dimethyl-1-naphthylamine is an aromatic amine and a dye.

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5,10,15,20-Tetraphenyl-21H, 23H-porphine-p,p′,p,p′′′-tetrasulfonic acid tetrasodium hydrate is a water-soluble porphyrin. It is a negatively charged compound due to the presence of the sulfonate groups. It has been used to functionalize chemically converted graphene to form conductive graphene films of varying thickness.

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3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4"-disulfonic acid sodium salt is also known as ferrozine.

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Mordant orange 1 is also known as Alizarin Yellow R, C.I. 14030. Mordant orange 1 is an azo dye. Azo dyes used as biological stains contain an azo group (−N=N−). It is used for colouring many different materials such as textile, leather, and plastics and for manufacturing paints and lacquers and for printings purposes as well.

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Water-soluble, negatively-charged spin trap used to monitor aqueous phase of SDS micelles.

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Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.