"Merck Millipore (Novagen)"

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Supplier: Merck Millipore (Novagen)

BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

Supplier: Merck Millipore (Novagen)

pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Supplier: Merck Millipore (Novagen)

Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

Supplier: Merck Millipore (Novagen)

The SpinPrep™ Gel DNA Kit enables efficient extraction of DNA fragments of 150 bp to >12000 bp in size from agarose gels. The procedure uses GelMelt™ solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, the purified DNA is eluted in low salt buffer. Each spin column can bind up to 20 µg DNA. Recoveries are consistently in the range of 50 to 90% under standard conditions.

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

Supplier: Merck Millipore (Novagen)

Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.

Supplier: Merck Millipore (Novagen)

The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

Supplier: Merck Millipore (Novagen)

NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and recA and endA mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Supplier: Merck Millipore (Novagen)

BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.