"EDVOTEK"
Experimentation kits, PCR-based VNTR human DNA typing
Supplier: EDVOTEK
In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. Using this kit, students type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.
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Do Onions, Strawberries and Bananas Have DNA?
Supplier: EDVOTEK
Kit allows students to construct DNA models and then extract DNA from onions, strawberries or bananas.
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Ready-to-Load™ kits, sickle cell gene detection (DNA-based)
Supplier: EDVOTEK
This Ready-to-Load™ kit shows how a restriction enzyme can be used to screen DNA for Sickle Cell Anaemia.
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Experimentation kits, amplification of DNA by PCR
Supplier: EDVOTEK
In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.
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Experimentation kits, simulation of HIV detection by western blot (agarose-based)
Supplier: EDVOTEK
The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.
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Transformation of E. coli with pGAL™
Supplier: EDVOTEK
In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.
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Micropipetting Basics
Supplier: EDVOTEK
Kit for teaching students how to use a micropipette with ease and accuracy using multi-coloured dyes.
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Pipette stand for six micropipettes
Supplier: EDVOTEK
Stand, pipette, Til: 6 pipetter
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UltraSpec-Agarose™, DNA electrophoresis grade
Supplier: EDVOTEK
Electrophoresis Gels, UltraSpec-Agarose™
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Horizontal Electrophoresis Apparatus, M6 and M12
Supplier: EDVOTEK
Capable of running one or two groups of student samples at the same time and producing excellent results in 30 to 40 minutes.
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Purification and size determination of green and blue fluorescent proteins
Supplier: EDVOTEK
When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
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Pipetting pump, electronic
Supplier: EDVOTEK
This lightweight, cordless pipetting controller is ideally suited as an aliquoting tool for instructors and teaching assistants. It uses all standard serological pipettes. The speed can be fine-tuned by applying varying finger pressure to the operating buttons.
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