2105 Resultater for: "Cytiva"
illustra™ TempliPhi™100/500 Amplification Kits
Supplier: Cytiva
TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
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Columns, PD SpinTrap™ G-25
Supplier: Cytiva
PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.
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Columns, PD MiniTrap™ G-25
Supplier: Cytiva
PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.
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HiLoad Superdex columns, 75 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
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Affinity chromatography columns, HiTrap™ TALON® crude
Supplier: Cytiva
HiTrap TALON® crude 1 and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.
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Amersham™ Protran® Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: Cytiva
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow
Supplier: Cytiva
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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illustra™ GFX™ PCR DNA and gel band purification kit
Supplier: Cytiva
The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.
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Western blotting blocking reagent, Amersham ECL™ Prime
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
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Percoll™ PLUS
Supplier: Cytiva
Percoll® PLUS is for preparation of cells, subcellular particles and larger viruses.
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Anti-IgG Donkey Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Accessories for ÄKTA chromatography systems
Supplier: Cytiva
On-line filter kit (20, 50, and 100 ml/min systems), PP, contains 10× filter, 2× support screen
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illustra™ MicroSpin™ S-400 HR columns
Supplier: Cytiva
illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.
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Blotting-membraner, Amersham Hybond™-N+
Supplier: Cytiva
Amersham™ Hybond™-N+ er en positivt ladet nylonmembran med en bindingskapacitet for nukleinsyrer på op til 600 µg/cm² og anbefales til brug sammen med radioaktive eller ikke-radioaktive kemiluminescensdetektionssystemer.
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Affinity chromatography media, Glutathione Sepharose™ 4B
Supplier: Cytiva
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.
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Blotting-membraner, Amersham Hybond™-XL
Supplier: Cytiva
Amersham™ Hybond™-XL er en positivt ladet nylonmembran, som er designet til at give optimale signal-støj-forhold ved brug sammen med radioaktivt mærkede prober. Amersham™ Hybond™-XL har en større koncentration og en mere ensartet fordeling af positiv ladning end andre ladede nylonmembraner. Den har bevaret alle egenskaberne fra andre nylonmembraner, herunder høj bindingskapacitet på op til 600 µg/cm², effektiv binding af små fragmenter, effektiv aftagning af probe samt fremragende egenskaber til gentagen undersøgelse.
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Empty columns, XK series
Supplier: Cytiva
XK empty columns are designed for liquid chromatography at low to medium pressure. The user friendly design ensures trouble-free operation with fittings for direct connection to high performance liquid chromatography systems. They are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules. They are not resistant to acetone, ktones, chlorinated hydrocarbons, aliphatic esters and phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid and strong mineral acids.
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Fraction collector, Frac30
Supplier: Cytiva
Frac30 is a round fraction collector designed for use with the ÄKTA start purification system. The Frac30 is controlled via the ÄKTA start touch screen instrument display or using UNICORN start software on a computer. The Frac30 is attached to ÄKTA start for power and communication. It is not suited for standalone operation.
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Pharmalytes® IEF carrier ampholytes
Supplier: Cytiva
Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
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Western blotting-detektionsreagens, Amersham™ ECL™ Prime
Supplier: Cytiva
Amersham ECL™ Prime er en meget følsom Western blotting-detektionsreagens, der kendetegnes ved stabil signalemission og tillader muligheden for gentagne eksponeringer samt gør det lettere at behandle flere blot i samme forsøgskørsel. Amersham ECL™ Prime leverer et detektionssystem, der er følsomt, stabilt, præcist kvantitativt inden for et bredt dynamisk område af proteinniveauer, og som sparer på forbruget af kostbare antistofreagenser. Amersham ECL™ Prime's høje ydeevne skyldes tilstedeværelsen af en forstærker i reagenset, der øger enzymomsætningen og dermed øger både signalintensitet og -varighed markant. Lyssignalet forstærkes yderligere af tilføjelsen af en katalysator.
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Accessories for empty columns HiScale™
Supplier: Cytiva
Union Valco F/F, PEEK, for HiScale™ 16 and 26
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Gel filtration media, Sephadex™ LH-20
Supplier: Cytiva
Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.
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illustra™ MicroSpin™ S-300 HR columns
Supplier: Cytiva
illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.
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Accessories for empty high performance columns Tricorn™
Supplier: Cytiva
Fingertight connector 1/16" male
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Percoll™
Supplier: Cytiva
Percoll® is commonly used for separating cells, organelles, viruses, and other subcellular particles.
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Blotting membranes, Amersham™ Hybond™ 0.2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ 0.2 PVDF is a 0,2 μm pore size hydrophobic membrane with high physical strength. This results in significant handling advantages over unsupported nitrocellulose and makes the membrane highly suitable for stripping and reprobing. The small pore size of the membrane minimises 'blow-through' and increases protein binding over a wide range of molecular weights. Ideal for use with standard colorimetric and chemiluminescent detection methods for proteins of
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Agarose IEF
Supplier: Cytiva
Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications.
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pI markers
Supplier: Cytiva
pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.