"Biotium"

Supplier: Biotium

RuBP-4S ([Ruthenium(bathophenanthroline)(bis(bathophenanthrolinedisulfonate))],disodium salt) is a luminescent ruthenium complex similar to RuBPS (80021). Two of the bathophenanthroline ligands are bis-sulfonated while the third bathophenanthroline ligand is neutral, resulting in an overall double negative charges of the ruthenium complex.

Supplier: Biotium

The principle of DPA/Tb3 for vesicle fusion assay is based on the fact that contact of the chelator dipicolinic acid (DPA) with terbium (III) forms an instant Tb3 -DPA complex that is ~10,000 times more fluorescent than free Tb3 . In the assay, separate vesicle populations are loaded with 2.5 mM TbCl3 in 50 mM sodium citrate, or 50 mM DPA in 20 mM NaCl.

Supplier: Biotium

Biotium developed SDIP/Eu3 as an alternative to DPA/Tb3 (80104), which is used for vesicle fusion assays. Similar to DPA/TB 3 assay, one population of vesicles can be loaded with the chelator SDIP while another population of vesicles can be loaded with EuCl3. Intense red fluorescence is produced when the two types of vesicles fuse as a result of formation of the SDIP/Eu3 complex.

Supplier: Biotium

The fluorescence of NBD is highly dependent on the environment; it is virtually nonfluorescent in water but becomes strongly green fluorescent in hydrophobic media. NBD-ethylenediamine can be readily coupled to activated carboxy groups or sulfonyl chlorides.

Supplier: Biotium

5-FAM-PEO8 SE (full name: 5-Carboxyfluorescein-PEO8 propionate succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates fluorescein and the amine-reactive SE group.

Supplier: Biotium

5-ROX-PEO8 SE (full name: 5-Carboxy-X-rhodamine-PEO8-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group.

Supplier: Biotium

Eosin-5-isothiocyanate is a good photosensitizer and can be used to selectively label amines.

Supplier: Biotium

Fluorescamine is nonfluorescent until it reacts with primary amines. The reaction is completed in miniseconds and can be used to detect amine-containing compounds including peptides and proteins. Unreacted fluorescamine is rapidly converted to a non-fluorescent product by reaction with water, making amine detection highly specific.

Supplier: Biotium

5-SFX (full name: 6-(Fluorescein-5-carboxamido)hexanoic acid, succinimidyl ester, single isomer) has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group.

Supplier: Biotium

6-SFX (full name: 6-(Fluorescein-6-carboxamido)hexanoic acid, succinimidyl ester, single isomer) has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group.

Supplier: Biotium

SR101-PEO8 SE (Sulforhodamine 101-PEO8-propionic acid succinimydyl ester; Texas Red®-PEO8 SE) has an approximately 32 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.

Supplier: Biotium

SR101-PEO12 SE (Sulforhodamine 101-PEO12-propionic acid succinimydyl ester; Texas Red®-PEO12 SE) has an approximately 47 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.

Supplier: Biotium

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.

Supplier: Biotium

Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

Supplier: Biotium

These antibody labelling kits include amine-reactive near-IR CF™ dye as well as all components required for antibody labelling and purification.

Supplier: Biotium

6-TAMRA SE (full name: 6-Carboxytetramethylrhodamine succinimidyl ester) is the amine-reactive form of Amine-reactive form of Carboxytetramethylrhodamine (TAMRA) single isomer. Supplied at 90 mg/mL (0.17 M) in anhydrous DMSO. The solution is suitable for labeling oligonucleotides.