"Biotium"

Supplier: Biotium

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

Supplier: Biotium

Difluoro BAPTA AM ester is a membrane permeable form of Difluoro BAPTA that can be loaded into cells by incubation. Difluoro BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Difluoro BAPTA by cytoplasmic esterases. See #50006 for information on Difluoro BAPTA.

Supplier: Biotium

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

Supplier: Biotium

Dimethyl BAPTA AM ester is a membrane permeable form of Dimethyl BAPTA that can be loaded into cells by incubation. Dimethyl BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Dimethyl BAPTA by cytoplasmic esterases.

Supplier: Biotium

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

Supplier: Biotium

The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.

Supplier: Biotium

Flubi-2 is the hydrolyzed product of the site-directed fluorogenic probe Flubida-2 (#51022) and can be used as a standard for calibration. The dye can also be microinjected into cells to probe the local pH where avidin fusion proteins are located.

Supplier: Biotium

MQAE (N-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide) is an improved chloride indicator that has greater sensitivity to chloride (Ksv= 200M-1) than SPQ (Ksv=118 M-1) and higher fluorescence quantum yield. The ester group of MQAE may slowly hydrolyze inside cells, resulting in a change in its fluorescence response.

Supplier: Biotium

Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 to facilitate cell loading.

Supplier: Biotium

Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca2 indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn2 to enter cells to quench indicator fluorescence. However, since 4-bromo A-23187 is nonfluorescent, it is preferred over the blue fluorescent A-23187 for calibrations of UV-excited Ca2 indicators such as Indo-1 and Fura-2 in order to keep background fluorescence to a minimum.

Supplier: Biotium

Ionomycin is commonly used to modify intracellular calcium concentrations and to calibrate fluorescent calcium indicators. It is also known for its antiproliferative effects.

Supplier: Biotium

Fluorescein-DHPE (N-(Fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) is a useful surface pH indicator (1) and has also been used for measuring lateral diffusion in membranes via fluorescence photobleaching recovery method.

Supplier: Biotium

NBD-PE (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt) in combination with Rhodamine-DHPE (#60026) or Texas Red®-DHPE (#60027) has been used to study membrane fusion via fluorescence resonance energy transfer (FRET) (1-3). NBD-PE has also been used in photobleaching recovery measurement.

Supplier: Biotium

Texas Red®-DHPE is a rhodamine-labeled glycerophosphoethanolamine lipid.

Supplier: Biotium

TRITC-DHPE is a rhodamine-labeled glycerophosphoethanolamine lipid. DHPE conjugates of rhodamine dyes have been used in membrane fusion assays.

Supplier: Biotium

NBD C6-ceramide (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine) has been used for following sphingolipid metabolism in cells, and as a selective stain for the Golgi apparatus in live and fixed cells.