"76177-196"

Supplier: Biosensis

The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (Aβ) associated with the pathology characteristic of Alzheimer’s disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for Aβ have been shown to actually detect intraneuronal APP and not Aβ exclusively.

MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide. MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice).

Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for Aβ40 and Aβ42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal Aβ, distinct from Aβ associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.

Biosensis now offers biotinylated MOAB-2 antibody allowing more flexibility in experimental design by using the biotin-avidin/streptavidin detection method. Biotinylated MOAB-2 antibody may also help to reduce background staining in difficult-to-stain tissues and increase detection sensitivity. The ability of biotinylated MOAB-2 antibody to detect amyloid beta has been validated by IHC.

Supplier: Abnova

HSPB8 RECOMBINANT PROTEIN (Q02) 1 * 1 Each

Supplier: Abnova

CDKL4 (HUMAN) RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

USP45 (HUMAN) RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

CRYBA4 (HUMAN) RECOMBINANT PROTEIN (P01) 1 * 1 Each

Supplier: Abnova

SERTAD3 RECOMBINANT PROTEIN (P01) 1 * 1 Each

Supplier: Abnova

NPIP RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

CD320 RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

CDKL4 RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

CLDN18 RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

EXOSC1 RECOMBINANT PROTEIN (P01) 1 * 1 Each

Supplier: Abnova

C13ORF1 RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

HAAO RECOMBINANT PROTEIN (Q01) 1 * 1 Each

Supplier: Abnova

RPL19 RECOMBINANT PROTEIN (P01) 1 * 1 Each

Supplier: Abnova

CLEC1B RECOMBINANT PROTEIN (P01) 1 * 1 Each

Supplier: Abnova

TEX261 RECOMBINANT PROTEIN (P01) 1 * 1 Each