Pfu DNA polymerase

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Pfu DNA Polymerase MasterMix (2X) is a premixed, ready to use solution containing Pfu DNA Polymerase, dNTPs, MgSO₄ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu MasterMix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination.

Unit definition: One unit (U) of Pfu Polymerase is defined as the amount of enzyme needed to catalyse the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70 °C using herring sperm DNA as a substrate.