N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

• Reduces mRNA immunostimulatory effects

  Improves mRNA translation efficiency

  Enhances mRNA stability

  ≥99% purity by AX-HPLC analysis

  Provided as 100 mM in H₂O, pH 7,5

  Grade: RUO

N1-Methylpseudouridine-5'-Triphosphate (N1MePsU) is a modified NTP for incorporation into messenger RNAs (mRNA) using an enzyme such as T7 RNA Polymerase. Modified uridines such as N1-methylpseudouridine, 5-methoxyuridine, and pseudouridine can reduce unwanted immunogenic response and enhance translational efficiency of mRNAs. These properties can result in safer mRNA and increased protein expression.