Trypsin-EDTA (0,05%)

Enzymatic cell dissociation reagent.

• Generate single-cell suspenstions of human ES and iPS cells for downstream assays with a gentle, low concentration Trypsin-EDTA solution

Generate single-cell suspensions of human embryonic stem cells (ES) or human induced pluripotent stem (iPS) cells for flow cytometry or cloning assays with Trypsin-EDTA (0,05%). EDTA weakens cell-cell adhesion, which improves trypsin's access to hydrolysis-targeted peptide bonds, and enhances detachment of adherent cells. This is a gentler version than the standard concentration (0,25%), and has applications to many other cell culture systems in addition to human ES cells and iPS cells.

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