Spermine ELISA Kit

Spermine ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Spermine in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-to-use ELISA kit
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Spermine ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Spermine in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Spermine antigen. During the incubation, Spermine present in the samples or standards competes with the fixed amount of immobilised Spermine for binding sites on the Biotinylated Anti-Spermine Antibody. The more Spermine present in a sample or standard, the less Biotinylated Anti-Spermine Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Spermine Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Spermine present in each sample or standard. The concentration of Spermine can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.