Mouse Trap1a ELISA Kit

Trap1a ELISA Kit (Mouse) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Trap1a has been pre-coated onto a 96-wellplate (12×8 well strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Trap1a is added, incubated and followed by washing. Avidin-Peroxidase conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Trap1a captured in well.

ELISA Kit Reproducibility:
Mean Intra-assay CV: <10%
Mean Inter-assay CV: <12%

Leveringsinformation: Kit includes Trap1a microplate, Trap1a lyophilized standard, 100X Biotinylated Trap1a detector antibody, 100X Avidin-HRP conjugate, sample diluent, detector antibody diluent, conjugate diluent, 25X wash buffer, stop solution and TMB substrate.