ClonaCell™-HY Medium E

Hybridoma growth medium with hypoxanthine and thymidine (serum-containing).

ClonaCell™-HY medium E is a serum-containing, nutritionally rich liquid medium optimised for hybridoma expansion after hypoxanthine, aminopterin, thymidine (HAT) selection. The medium contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin used during the selection process. This medium has been verified for use in mouse and rat hybridoma development and monoclonal antibody production and reportedly is compatible for production, cloning, and expansion of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster. ClonaCell™-HY medium E has proven effective for subcloning and stabilisation of unstable hybridoma cell lines.

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