Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.

• Naturally-secreting Cypridina luciferase gene, optimised for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimise background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells respectively
• High-copy pUC bacterial DNA replication origin

pMCS-Cypridina Luc vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Cypridina luciferase reporter.

pCMV-Cypridina vector has the luciferase gene under the CMV (Cytomegalovirus) promoter. Strong (CMV) constitutive promoters for co-transfection and normalisation.

pTK-Cypridina vector has the luciferase gene under the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. Weak (TK) constitutive promoters for co-transfection and normalization.

The gene encodes a naturally-secreted bioluminescent Cypridina luciferase (62 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.