Purified DNase I tested to be free of contaminating activity for the degradation of nucleic acids.

• RNase free
• Contains a mixture of four isoenzymes from bovine pancreas (10 units/µl)

DNase I, RNase Free catalyses the degradation of double-stranded DNA into oligonucleotides 1,2 and mononucleotides. This enzyme has been isolated as a mixture of four isoenzymes from bovine pancreas that cut preferentially next to pyrimidine nucleotides.

Reaction Conditions: 40 mM Tris-HCl (pH 7,5), 6 mM MgCl₂, 2 mM CaCl₂, 1 µg DNA and 1 unit enzyme in a 25 µl volume. Incubate 30 minutes at 37 °C.